The biorefining technologies were scaled up to produce bioethanol, biobutanol and biohydrogen with competitive production costs. The pretreatment capacity was up to one ton of biomass (dry basis) per day. The bioethanol production capacity was 50 litres per day. The environmental and economic sustainability of the proposed technologies were analysed, and technology transfer mechanisms were established.

A conceptual design of a biorefinery for 10 ton (DB)/day of biomass was produced identified as a “mini-biorefinery”. Other designs for 100 to 600 ton (DB)/day feedstock were also produced with the following characteristics:

• Coproduction schemes of advanced biofuels (bioethanol, biobutanol and biohydrogen) and carbon dioxide
• Us of residual lignin for producing high-added value products 
• Minimum carbon emissions and minimum water usage 
• Use of low-investment technologies 
• Total production costs of 0.45 USD per litre of advanced bioethanol
• Environmental sustainability analisys